Resources

Find out how to take the most out of our software and see what our team is working on.

Cases of use

Global Analysis in Isothermal Titration Calorimetry Displacement Titrations with AFFINImeter
Isothermal titration calorimetry (ITC) displacement titrations offer an attractive alternative to standard ITC assays when working with ultra high- or ultra low- affinity interacting systems. The method requires the fitting of at least two isotherms that share various adjustable parameters. AFFINImeter counts with advanced tools, like the global fitting of multiple dataseries, which can facilitate the analysis and expand the range of applications of ITC experiments like the displacement titration.

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Analytical discrimination of ligands in heterogeneous mixtures with AFFINImeter
It is not uncommon that the concentration of one or more of the compounds involved in a binding study is not accurately determined. Furthermore, researchers dealing with a mixture of isomers that bind to the same target often assume that there is one single compound in the sample. Under those conditions, fitting ITC isotherms to a 1:1 binding model leads to a completely wrong interpretation of the data. AFFINImeter can be used as an efficient analytical tool to globally analyze sets of ITC isotherms of two competitive ligands that bind the same target, even if their concentration is unknown.

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Solving complex interactions with AFFINInimeter: Competing ligands binding to a multiple site receptor
Isothermal Titration Calorimetry (ITC) is a versatile technique with the potential of deconvoluting the various binding events that may coexist in complex interactions. In this sense, a major drawback has been the lack of mathematical models and computational tools to properly analyze such experiments. AFFINImeter counts with an advanced functionality, the "Model Builder" with which researchers can easily design their own binding models through the combination of distinct (coupled) binding equilibria, to obtain thermodynamic and structural information from complex ITC experiments.

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Video Tutorials

Our scientific team recorded these tutorials to help you get started with AFFINImeter.
You can see them all in our Youtube Channel
KinITC for TA Instruments Calorimeters
HOW to export TA files into AFFINImeter
HOW TO prepare a FIT subproject
HOW TO simulate ITC isotherm
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Webinars

These videos are recorded broadcasts of our latest workshop: Widen-ITC 2014.
Also, on the workshop webpage you can find all the information related to the workshop.

Guest presenters at Malvern Webinar

Philippe Dumas
University of Strasbourg
Kinetic ITC sheds light on RNA-mediated genetic regulation
Natalia Markova
Malvern Instruments Limited
Good quality ITC data: experimental design and data analysis

Table of representative binding models

Basic predefined models
NAME REACTION SCHEME DESCRIPTION EXPERIMENTAL SETUP (*)
1:1 Simple model 1:1 Simple model 1:1 Simple modelTwo species form a 1:1 complex (I)
A=species 1
M=species 2
Dimer Dimer DimerOne species auto-associate to form a dimer (II)
A=single species
Dimer – trimer Dimer – trimer Dimer – trimerStepwise auto-association to form a dimer and a trimer (II)
A=single species
1:1 Competitive 1:1 Competitive 1:1 CompetitiveTwo competing ligands bind to the same receptor (III), (IV), (V)
A= ligand 1
B= ligand 2
M=receptor
1:1 Competitive reverse 1:1 Competitive reverse 1:1 Competitive reverseTwo competing ligands bind to the same receptor (III), (IV), (V)
A= receptor 1
M=ligand 1
B= ligand 2