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Getting Started with AFFINImeter
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Also, you will get some answers to some common (and not so common) questions regarding using ITC, AFFINImeter, etc..
Cases of use
Global Analysis in Isothermal Titration Calorimetry Displacement Titrations with AFFINImeter
Isothermal titration calorimetry (ITC) displacement titrations offer an attractive alternative to standard ITC assays when working with ultra high- or ultra low- affinity interacting systems. The method requires the fitting of at least two isotherms that share various adjustable parameters. AFFINImeter counts with advanced tools, like the global fitting of multiple dataseries, which can facilitate the analysis and expand the range of applications of ITC experiments like the displacement titration.
View more | Download PDF | View ISSU Analytical discrimination of ligands in heterogeneous mixtures with AFFINImeter
It is not uncommon that the concentration of one or more of the compounds involved in a binding study is not accurately determined. Furthermore, researchers dealing with a mixture of isomers that bind to the same target often assume that there is one single compound in the sample. Under those conditions, fitting ITC isotherms to a 1:1 binding model leads to a completely wrong interpretation of the data. AFFINImeter can be used as an efficient analytical tool to globally analyze sets of ITC isotherms of two competitive ligands that bind the same target, even if their concentration is unknown.
View more | Download PDF | View ISSU Solving complex interactions with AFFINInimeter: Competing ligands binding to a multiple site receptor
Isothermal Titration Calorimetry (ITC) is a versatile technique with the potential of deconvoluting the various binding events that may coexist in complex interactions. In this sense, a major drawback has been the lack of mathematical models and computational tools to properly analyze such experiments. AFFINImeter counts with an advanced functionality, the "Model Builder" with which researchers can easily design their own binding models through the combination of distinct (coupled) binding equilibria, to obtain thermodynamic and structural information from complex ITC experiments.