Solving complex interactions with AFFINInimeter: Competing ligands binding to a multiple site receptor

Posted on Posted in Cases of use, ITC

Isothermal Titration Calorimetry (ITC) is a versatile technique with the potential of deconvoluting the various binding events that may coexist in complex interactions. In this sense, a major drawback has been the lack of mathematical models and computational tools to properly analyze such experiments. AFFINImeter counts with an advanced functionality, the “Model Builder” with which […]

Global Analysis in Isothermal Titration Calorimetry Displacement Titrations with AFFINImeter

Posted on Posted in Cases of use, ITC

Isothermal titration calorimetry (ITC) displacement titrations offer an attractive alternative to standard ITC assays when working with ultra high- or ultra low- affinity interacting systems. The method requires the fitting of at least two isotherms that share various adjustable parameters. AFFINImeter counts with advanced tools, like the global fitting of multiple dataseries, which can facilitate […]