What is an AFFINImeter Basic Plan?
The basic plan is recommended for beginners and experienced ITC users working with systems that follow traditional stoichiometric binding models. This plan comes with a list of predefined models ready to apply for data for analysis. Easier and faster than using a conventional software it also includes KinITC, a new method to obtain kinetic information from ITC data. With one single titration experiment, it calculates rate constants (kon and koff) and thermodynamic data (KA and DH) of 1:1 and 1:n interactions.
List of Available stoichiometric models for this plan:
1. Stoichiometric models
2.Independent Sites Model
Considers a receptor (in the cell) with “N” sets of “n” independent sites binding to a ligand (in the syringe) or to two competing ligands.
What is an AFFINImeter Advanced Plan?
The advanced plan is recommended for beginners and experienced ITC users working with complex interacting systems that don´t follow conventional binding modes. This plan includes all the features of the basic plan plus advanced tools of analysis: it offers the predefined models available in the basic plan and also the tool “model builder” with which users can create tailored binding models in an easy and straightforward way. The advanced plan includes the possibility to perform global fitting of multiple isotherms.
An example of a tailored binding model that can be generated with this plan:
This model represents the interaction of two competing ligands (A and B) with a receptor (M) with three binding sites. Contact us for more information on how to design binding models with the model builder.
What is an AFFINImeter Advanced Team Plan?
The Advanced team plan is recommended for research groups/core facilities with many ITC users. This plan consists of a multi-user license comprising one administrator account and two or more standard user accounts. The administrator (i.e. the head of the research team or core facility) can visualize and edit its own account and the other user accounts. All the accounts of the team plan contain the tools of the Advanced Plan.
Who needs a local installation?
By default, AFFINImeter is provided as a cloud software and local installations are available only upon request. AFFINImeter on the cloud offers many advantages like no need of software installation and right away access to your account and data analysis from any computer/tablet/smartphone connected to the internet. The option of a local installation is requested i.e. when researchers are not authorized by their institution to upload data to the internet.
What are the instruments supported in AFFINImeter?
Microcal VP-ITC 1.4 mL
Microcal ITC-200 0.2 mL
TA Nano-ITC Low Volume 0.19 mL
TA Nano-ITC Standard 1 mL
TAM III – TA Instruments