Analytical discrimination of ligands in heterogeneous mixtures with AFFINImeter

Posted on Posted in Cases of use, ITC

It is not uncommon that the concentration of one or more of the compounds involved in a binding study is not accurately determined. Furthermore, researchers dealing with a mixture of isomers that bind to the same target often assume that there is one single compound in the sample. Under those conditions, fitting ITC isotherms to a 1:1 binding model leads to a completely wrong interpretation of the data. AFFINImeter can be used as an efficient analytical tool to globally analyze sets of ITC isotherms of two competitive ligands that bind the same target, even if their concentration is unknown.