Cases of use
Solving complex interactions with AFFINInimeter: Competing ligands binding to a multiple site receptor.
Isothermal Titration Calorimetry (ITC) is a versatile technique with the potential of deconvoluting the various binding events that may coexist in complex interactions. In this sense, a major drawback has been the lack of mathematical models and computational tools to properly analyze such experiments. AFFINImeter counts with an advanced functionality […].
Analytical discrimination of ligands in heterogeneous mixtures with AFFINImeter.
It is not uncommon that the concentration of one or more of the compounds involved in a binding study is not accurately determined. Furthermore, researchers dealing with a mixture of isomers that bind to the same target often assume that there is one single compound in the sample. Under those conditions, fitting ITC isotherms to a 1:1 binding model leads to a completely wrong interpretation of the data. AFFINImeter can be as an […].
Global Analysis in Isothermal Titration Calorimetry Displacement Titrations with AFFINImeter.
Isothermal titration calorimetry (ITC) displacement titrations offer an attractive alternative to standard ITC assays when working with ultra high- or ultra-low- affinity interacting systems. The method requires the fitting of at least two isotherms that share various adjustable parameters. AFFINImeter counts with advanced tools, […].
The course of Isothermal Titration Calorimetry data analysis
Isothermal Titration Calorimetry (ITC) is widely considered the gold standard technique for the direct determination of binding thermodynamics.
Nowadays, getting kinetic information directly from the thermogram is also possible thanks to the method KinITC implemented in AFFINImeter. Besides KinITC, AFFINImeter offers a number of advanced analytical tools to take the most out of ITC data.
Aiming to show researchers how to make use of these tools, we have prepared a course on ITC data analysis, consisting of a series of video tutorials imparted by Eva Muñoz (Senior Scientist at AFFINImeter). You can visualize the full course by clicking on the button: